uMRT cDNA Synthesis & Amplification Kit

The UltraMarathonRT® (uMRT) cDNA Synthesis & Amplification Kit enables you to see more of the transcriptome in each sample. uMRT is a next-generation reverse transcriptase that synthesizes end-to-end cDNA in a single pass from even the most complex RNA molecules—long, structured, high-GC, or low-abundance. Conventional retroviral RTs are ‘distributive’ meaning they attach and detach many times from each RNA transcript which often creates truncated, incomplete, or incorrect cDNAs. This is often caused by secondary structures or repeat sequences within the template. UltraMarathonRT solves this problem. It is a highly engineered group II intron-encoded reverse transcriptase with intrinsic helicase activity. It bypasses the drop-offs, biases, and blind spots common in traditional cDNA synthesis workflows. Why Choose This Kit Higher Gene Detection & Isoform Sensitivity Captures more genes and isoforms, including alternative splicing and intron retention events that other kits miss. Superior Amplification Accuracy Uniform gene body coverage improves the accuracy of differential gene expression analysis. Designed for Modern RNA-Seq Applications Perfect for short-read sequencing, biomarker discovery, and therapeutic target validation. Applications Bulk RNA-Seq Short-read RNA-Seq Low-abundance transcript detection Isoform-level resolution Alternative splicing analysis RNA biomarker discovery UltraMarathonRT cDNA Synthesis & Amplification Kit Components: UltraMarathonRT (20 U/µL) 2x RT Buffer S dNTP mix (10 mM each) dATP solution (10 mM) uMRT oligo(dT) primer (5 μM) uMRT-TSO_v2 AmpPCR 5x Template Switching Buffer Nuclease-free water 5x DNA Polymerase Buffer DNA Polymerase (1 U/µL) Reagents Required but Not Supplied: RNA template: 1 ng – 100 ng total cellular RNA or 0.1 ng – 20 ng mRNA is recommended for each reaction. Post PCR clean-up reagent. Product Information Catalog# R1007S (20 reactions), R1007M (50 reactions), R1007L (100 reactions) Storage Conditions: For short duration of storage (< 6 month), UltraMarathonRT can be stored in a -20℃ freezer. -80℃ is recommended for long-term storage. All other kit components are stable in a -20℃ freezer for long-term storage. Note that UltraMarathonRT can endure 10 freeze-thaw cycles without losing noticeable activity. After 20 freeze-thaw cycles, UltraMarathonRT maintains 90% of its original activity. For Research Use Only.