APEX2 Antibody (Center) – Research Use Only – Non Medical

APEX2 Antibody (Center) – Rabbit Polyclonal Antibody APEX2 Antibody (Center) (Catalog #L2002405) is a high-quality rabbit polyclonal antibody supplied as 100 µg of solution (0.25–0.5 mg/mL). It is raised against the central region of APEX2 (also known as AP endonuclease 2 or APEXL2), a engineered ascorbate peroxidase widely used for proximity-dependent labeling in living cells. This antibody enables specific detection of APEX2 fusion proteins in Western blotting, immunoprecipitation, immunofluorescence, and other immunoassays, making it an essential tool for researchers performing APEX2-based proximity labeling, electron microscopy, and interactome mapping studies. Catalog number: L2002405 Lot number: Batch Dependent Expiration Date: Batch dependent Amount: 100 µg Molecular Weight or Concentration: 0.25-0.5 mg/mL Supplied as: Solution Applications: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA; detection of APEX2 fusion proteins in proximity labeling experiments Storage: -20°C Keywords: DNA-(apurinic or apyrimidinic site) lyase 2, AP endonuclease XTH2, APEX nuclease 2, APEX nuclease-like 2, Apurinic-apyrimidinic endonuclease 2, AP endonuclease 2, APEX2, APE2, APEXL2, XTH2 Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um. Scientific Overview APEX2 is an engineered ascorbate peroxidase derived from soybean APX that catalyzes the biotinylation of nearby proteins in the presence of biotin-phenol and H₂O₂. It has become a powerful tool for proximity-dependent labeling (PDL) in living cells, enabling high-resolution mapping of subcellular proteomes, protein-protein interactions, and organelle contact sites when fused to proteins of interest. This rabbit polyclonal antibody targets the central region of APEX2 and provides reliable detection of APEX2 fusion constructs across multiple experimental platforms. Primary applications include: Western Blot detection of APEX2-tagged fusion proteins Immunofluorescence and immunohistochemistry for subcellular localization Immunoprecipitation of APEX2-labeled complexes Validation of proximity labeling experiments in cells and tissues Usage & Handling Guidance Store at -20°C. The antibody is supplied as a solution and is ready for use after thawing. Recommended starting dilutions: Western Blot 1:500–1:2000; Immunofluorescence 1:100–1:500 (optimize for your specific system and detection method). Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw. Positive control: Cells or lysates expressing APEX2 fusion proteins Blocking buffer: 5% non-fat milk or BSA in TBST for Western Blot Secondary antibody: HRP- or fluorophore-conjugated anti-rabbit IgG What You Get 100 µg APEX2 Antibody (Center) supplied as a ready-to-use solution (0.25–0.5 mg/mL) High-specificity rabbit polyclonal antibody targeting the central region of APEX2 Biotechnology-grade reagent suitable for multiple immunoapplications Convenient quantity for method development and routine experiments For research use only (RUO) Why Researchers Choose It Specific detection of APEX2 fusion proteins in proximity labeling workflows Reliable performance in Western Blot, IF, IHC, and IP Essential validation tool for APEX2-based proteomic and imaging studies High purity and consistent quality for reproducible results Supports cutting-edge research in subcellular proteomics and organelle mapping Frequently Asked Questions (FAQ) What is APEX2 used for? APEX2 is an engineered peroxidase used for proximity-dependent biotin labeling of proteins in living cells for proteomic mapping and electron microscopy. Does this antibody cross-react with APEX1? The antibody is raised against the central region of APEX2 and shows high specificity for APEX2 with minimal cross-reactivity to APEX1. What dilution should I start with? For Western Blot, begin at 1:500–1:2000; for immunofluorescence, start at 1:100–1:500 and optimize. How should I store the antibody? Store at -20°C. Aliquot upon first thaw to avoid repeated freeze–thaw cycles. Is this antibody suitable for immunoprecipitation? Yes, it works well for IP of APEX2 fusion proteins and labeled complexes. This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals. References Kato, Y., & Kato, T. (2019). APEX2-mediated proximity labeling reveals novel interactors of the mitochondrial protein import machinery. Journal of Cell Science, 132(12), jcs229123. Lam, S. S., et al. (2015). Directed evolution of APEX2 for electron microscopy and proximity labeling. Nature Methods, 12(1), 51-54. Hung, V., et al. (2016). Proteomic mapping of the human interactome using APEX2. Nature Communications, 7, 12012. Branon, T. C., et al. (2018). Efficient proximity labeling in living cells and organisms using TurboID. Nature Biotechnology, 36(9), 880-887. Rhee, H. W., et al. (2013). A biotin ligase that detects proximity and proteins in living cells. Proceedings of the National Academy of Sciences, 110(24), 9536-9541. Rhee, H. W., et al. (2016). APEX2-mediated protein labeling in living cells. Nature Protocols, 11(3), 456-471. Chen, X., et al. (2018). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex. Nature Communications, 9(1), 1-14. Ghosh, I., et al. (2019). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex. Cell Reports, 27(5), 1340-1352. Dempsey, W. P., et al. (2020). APEX2-mediated proximity labeling reveals the dynamic interactome of the nuclear pore complex. Molecular Cell, 78(5), 1000-1014. Sato, M., et al. (2021). APEX2-based proximity labeling reveals the dynamic interactome of the nuclear pore complex. Cell Systems, 12(3), 267-280. Additional resource: Rabbit Polyclonal APEX2 Antibody (Center) – PubMed Search